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| Author: Aaron Hall |
Several U.S. funding agencies provide support for cDNA-related projects. To annotate developing chromosome maps, DOE in 1990 began dedicated support for improved cDNA library production, early EST generation by J. Craig Venter's team at TIGR, physical mapping of cDNAs onto chromosomes, and database support. High-throughput correlations of cDNAs with the new BAC resources are also in progress. Sequencing of cDNAs corresponding to genes recognized during genomic sequencing is often a component of major chromosome sequencing projects.
The NIH National Human Genome Research Institute also is supporting research and development in cDNA library improvement and mouse cDNA library production. In the NIH-supported chromosome map development using radiation hybrid methodologies, about one-third of the markers are derived from ESTs. The source genes thus are mapped onto the chromosomes. Recently the NIH National Cancer Institute (NCI) began providing substantial support for cDNA library production and analysis in a major effort to identify cancer-related genes [see article, HGN 8(3-4), 8]. This effort, called the Cancer Genome Anatomy Project, CGAP, was described at the workshop by Carol Dahl and Robert Strausberg of NCI.
The Merck Genome Research Institute supports programs to characterize cDNAs representing disease genes, including full-length cDNA cloning and sequencing. The mouse model's usefulness for studying human diseases is being advanced with diverse collaborative support, including that from NIH for library construction and from DOE for I.M.A.G.E. efforts at LLNL to array mouse cDNA libraries. Washington University (St. Louis) generates mouse ESTs for the clone arrays with support from the Howard Hughes Medical Institute. |
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